A SIMPLIFIED PROCEDURE FOR THE ISOLATION OF HYPOPHYSEAL GROWTH HORMONE
نویسندگان
چکیده
منابع مشابه
Ultracentrifugation of hypophyseal growth hormone.
Sedimentation velocity was measured in a Spinco ultracentrifuge’ operating at 165,000 X g. The hormone solutions (0.5 per cent) were prepared with borate buffer of pH 9.7 or saline at pH 9.1. Table I presents a typical run in a borate buffer; the sedimentation velocity constant was calculated by the procedure described by Svedberg and Pedersen (3). It may be noted that the individual values of ...
متن کاملThe Hypophyseal Growth Hormone, Nature and Actions
many formulae and figures. Its many chapter subheadings give the book its rather terse, outline form. A discussion of methods of isolation, characterization, and determination of purity is followed by successive chapters dealing with reaction rates as a function of substrate concentration, inhibitor action, temperature, and pH. Thermodynamic concepts are well developed in chapters on energetics...
متن کاملSedimentation behavior of hypophyseal growth hormone.
In a previous publication, it was reported that solution of the hypophyseal growth hormone in acetate buffers results in an increment of its intrinsic viscosity with an accompanying increase of molecular weight (1). Later, Smith et al. (2) confirmed these observations in ultracentrifugal studies. The purpose of this present investigation has been to study the sedimentation behavior of the hormo...
متن کاملSimplified mammalian DNA isolation procedure.
The reverse genetics technologies that have recently been developed for mice have provided new tools to probe gene function in vivo. Unfortunately these powerful systems often require the analysis of large numbers of DNA samples. The genetargeting technology requires screening of embryonic stem-cell clones and later of the mice themselves, the latter also being the case for standard transgenic ...
متن کاملA Simplified Protocol for the Purification of Schwann Cells and Exosome Isolation from C57BL/6 Mice
Background: The purification of Schwann cells has proven to be a difficult process, with most methods requiring the use of special equipment. However, obtaining a sufficient number and high purity of Schwann cells is an integral aspect in their use for clinical application. Therefore, the aim of this study was to establish a simple and effective protocol for the isolation and purification of Sc...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1954
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)71145-9